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The overall effect of Taxol is to decrease the critical concentration of microtubule protein necessary for microtubule assembly. At a Taxol concentration of 5 M, the critical concentration of tubulin required for assembly decreases by a factor of 20 from 0.2, to less than 0.01 mg/ml. Taxol also affects the structure of the microtubule polymer by reducing the number of protofilaments from a normal average of 13 to 12 (Diaz et al., 1998).